NOA-NMD™ are steric hindrance antisense oligonucleotides that repress the target transcript abundance by nonsense-mediated decay (NMD). They are designed to disrupt the transcript reading frame, resulting in multiple premature termination codons in a manner that induces the NMD machinery. The interaction between complexes at the exon-exon junction (UPF2 and UPF3) and at the translation termination (UPF1) mediates target transcript degradation by the exoribonuclease enzyme..
Every base and backbone of our product is modified chemically to enhance its intracellular stability and binding specificity. This is possible as NOA-NMD™ mechanism of action is non-catalytic – they do not interact directly with any intracellular components in the NMD process. Consequently, NOA-NMD™ shows superior specificity, reproducibility and dose-response than siRNA and gapmer, which has limited capacity for chemical modifications. Doing so however, will abrogate the respective requisite interactions between siRNA and Dicer/RISC and between gapmer and RNase-H to effect transcript degradation.
NOA-Switch™ are steric hindrance antisense oligonucleotides for inducing a specific splice-event. Our products are designed for inducing exon skipping, exon inclusion, usage of alternate 3’ and 5’ splice sites beta, usage of mutually exclusive exons beta, intron retention and restoration beta. When compared to genetic-manipulation based approaches, our products with their relatively simple protocol will save you significant time and effort.
You may like to use our TranscriptomeViewer to visualize and compare the splicing events among various transcripts for the gene of your interest. We have make it easy for you to order the NOA-Switch™ products that are relevant to your study seamlessly through our viewer. Or you can contact us directly to order.
Usage : (i) investigate transcripts and splice-events functions after RNA-Sequencing or (ii) phenotype-based validation of drug targets
Options : pathway-centric, gene-family, and customized panels of NOA-Switch™ and NOA-NMD™
The commoditization of next-generation sequencing platforms allows routine characterization of transcripts abundance, and alternate/aberrant splicing events in the transcriptome. It is however, been very difficult to follow up with functional studies to identify specific splice-events/transcripts that regulate the cellular phenotype under study, be it normal cell physiology or disease pathology, in a practicable way.
Our functional study panels make it feasible to interrogate multiple splice-event/transcript candidates simultaneously and efficiently. They can be assembled accordingly to your RNA-Seq results, or to specific pathways and gene-families of your choice; depending on your candidates, the panels will consist of NOA-Switch™, NOA-NMD™ or a combination of both product groups. The oligonucleotides can be plated easily on a multi-well cell-culture plate for multiplex functional studies.